The Genome Sequence of the Tomato-Pathogenic Actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 Reveals a Large Island Involved in Pathogenicity

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The Genome Sequence of the Tomato - Pathogenic Actinomycete Clavibacter 1 michiganensis subsp . michiganensis NCPPB 382 Reveals a Large Island

4 KARL-HEINZ GARTEMANN, BIRTE ABT, THOMAS BEKEL, ANNETTE BURGER, 5 JUTTA ENGEMANN, MONIKA FLÜGEL, 1 LARS GAIGALAT, ALEXANDER 6 GOESMANN, INES GRÄFEN, JÖRN KALINOWSKI, OLAF KAUP, OLIVER 7 KIRCHNER, LUTZ KRAUSE, BURKHARD LINKE, ALICE McHARDY, FOLKER 8 MEYER,‡ SANDRA POHLE, CHRISTIAN RÜCKERT, 2,3 SUSANNE SCHNEIKER, EVA9 MARIA ZELLERMANN, ALFRED PÜHLER, RUDOLF EICHENLAUB,* OLAF 10 KAISER, AND DANIE...

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Evidence for plasmid-encoded virulence factors in the phytopathogenic bacterium Clavibacter michiganensis subsp. michiganensis NCPPB382.

The tomato pathogen Clavibacter michiganensis subsp. michiganensis NCPPB382, which causes bacterial wilt, harbors two plasmids pCM1 (27.5 kb) and pCM2 (72 kb). After curing of the plasmids, bacterial derivatives were still proficient in the ability to colonize the host plant and in the production of exopolysaccharides but exhibited a reduced virulence. When one of the two plasmids is lost, ther...

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A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants

Clavibacter michiganensis, subsp. michiganensis is a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum). Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors of C. michiganensis subsp. michiganensis and host-pathogen interactions on a molecular level are rather limited. In the study presented here...

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Linear plasmid in the genome of Clavibacter michiganensis subsp. sepedonicus.

Contour-clamped homogeneous electric field gel analysis of genomic DNA of the plant pathogen Clavibacter michiganensis subsp. sepedonicus revealed the presence of a previously unreported extrachromosomal element. This new element was demonstrated to be a linear plasmid. Of 11 strains evaluated, all contained either a 90-kb (pCSL1) or a 140-kb (pCSL2) linear plasmid.

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Bioluminescence imaging of Clavibacter michiganensis subsp. michiganensis infection of tomato seeds and plants.

Clavibacter michiganensis subsp. michiganensis is a Gram-positive bacterium that causes wilting and cankers, leading to severe economic losses in commercial tomato production worldwide. The disease is transmitted from infected seeds to seedlings and mechanically from plant to plant during seedling production, grafting, pruning, and harvesting. Because of the lack of tools for genetic manipulati...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 2008

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.01595-07